This paper experimentally investigates the effect of coating thickness on the thread, bearing friction coefficients, and torque-tension relationship in threaded fasteners, as well as an investigation into the effect of coating thickness on surface roughness properties. The torque-tension relationship is highly sensitive to frictional changes. Two different coating thicknesses are investigated using two bolt thread pitch; test data are collected for a preselected level of bolt tension. The experimental setup collects real-time data on the tightening torque, bolt tension, and the corresponding reaction torque. Test data are used for calculating the thread and bearing friction coefficients, as well as the overall torque-tension relationship for two different coating thicknesses. The study would provide an insight into the variation in the torque-tension relationship, which is a key factor that significantly affect the reliability and safety of bolted assemblies in many mechanical and structural applications.